Mitchell,
If I'm not mistaken, you're using Nebulosity, eh? You should be able to do all that pre-processing in Nebulosity.
Try it out the next time you're out doing your star trails. I think you'll find the calibration images will improve your already good star trails.
Improvements will include removing arbitrary noise (amp glow, read noise, thermal noise) and will also remove the strong vignetting which is often found on wide-angled lenses such as the one you're using.
My recommendation: take about an hour's worth of dark frames immediately after shooting your light frames. For flat frames, make sure you don't move the focus ring on your lens; the optical path has to match what your light frames were gathered with, as well as the same f/ratio. Load up Microsoft Paint, or any other application, which will allow you to have a pure white background on your screen. Rest your camera on the keyboard or somewhere else in front of your screen. Ensure your ISO is set to ISO-100, put your camera in Av (aperture priority) mode and set it to the same aperture as what your light frames were taken with, and snap off a couple of frames. Check the histogram on the LCD after each frame. What you want to do is see your histogram peak between 1/2-way and 2/3rd's of the way across the histogram. Once you think you've got the right exposure, take about 15 flat frames. Put the lens cap on your camera, make sure the viewfinder is covered on the back, and take 15 flat dark frames.
The rest should be a matter of loading the right files into Nebulosity (I'm not sure how to do this as I don't use Nebulosity for pre-processing) to create your master flat dark, master flat, master dark and so on.
Give it a go.
Regards,
Humayun