Hi John
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Originally Posted by JohnH
1. What is the best ISO setting to use for DSOs - ISO 1600 would appear to be best - but I see folks using 800, 400 even talk of 200. What advantage do the lower ISOs bring?
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There has been much discussion about the optimal ISO. The definitive work was done by Terry Lovejoy from Queensland.
http://www.pbase.com/terrylovejoy/dy...itivity_curves
As you will see it is a trade off between sensitivty and dynamic range.
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2. I appear to have more pronounced vignetting on the longer, lower ISO shots, is that normal - so I need to take flats now something I have not done yet. To do this I should shoot a clear dawn sky at ISO100 and a fast exposure and greyscale the result, yes?
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Yes that is one method. Lower ISO's in the flats reduce noise. You want as little noise as possible in your flats so as to not "add" more noise to your light frames. I usually use around 20 frames to make a master flat. The greyscaling will depend on what version of IP you have. Earlier versions required you to greyscale the flat before calibration, but the latest version (2.75) does this for you automatically. I take my flats indoors in daylight with a white cloth over the objective and the camera on P mode.
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3. Darks and flats must be taken (RAW or JPEG) to match the lights ?
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As Striker said (gosh he is right again

), take everything in RAW. Don't even think of JPEG.
Cheers