I assumed that may be the case with the darks Tom as I took some at the beginning, half way and towards the end and I noticed the initial ones were no where near dark must be something to do with sensor warm up
I'm starting to get some AMP glow in the bottom right corner don't know whats causing this may be the warmer weather
Your images are getting better and better. The guiding and stars look fantastic.
Would love to see you add many, many hours on this. You don't necessarily have to do a mosaic; just concentrating on the one region such as you have, really brings out the cluster amongst the beautiful nebulosity.
And, seriously, make sure you take darks after the session, and, also, start flat framing if you haven't already.
These little things will do wonders for your images.