Mike,
I find it the norm that, from my simplistic terminology, the dark frames for my DSLR will "subtract too much". I do my dark frame subtraction manually in photoshop, and find if there is a problem, it's easy to fade the subtraction (only subtract 80% for example), and this gets rid of them for me. There's always a balance where there's no significant number of dark specs left.
I'd say about 65% of the time I would need to fade the subtraction of dark frame, the rest of the time it's good enough.
I put it down to the difference in time between taking the images and taking the dark frames. I usually take a dark frame about once every hour.
With the ST7 there's often a few specs (say, 10) that I clone out once I've finished processing the image. Problem is no where near as bad with the ST7 presumably because of it's lower noise level and it's stable temperature.
It's one of those things.... 99.99% of the time people only ever show their good processed images, so unless you're "in the game" doing the imaging, you don't know just how the raw images and the rest of the processed images that the people don't show, turn out
Roger.