Thanks Nick & Dan, I'm glad that this has helped out the both of you as well. It is a testament to the great spirit of this forum.
I managed another imaging run on M16 again last night. This is a combination of 50 images @ 21.2 seconds with the DSI II Pro and the f/3.3 focal reducer. The gain was set to 0 this time and darks were subtracted.
The stars have now sharpened considerably which is very pleasing however I now have noticed that there are what looks like record player grooves appearing, especially to the right of the nebula.
I'm not sure what has caused it as it was not present in the preview when taking the subs only after combining. I have not encountered this before so I'm a bit clueless as to what it is. A few ideas that have sprung to mind is that the image was taken very close to a 98% Moon so that might have had an effect or now that the gain is at 0 should I have adjusted the offset as well which was set to 50.
All thoughts and comments appreciated.
Cheers all