JP, you shouldn't require the T Adapter for prime focus, just the T Ring to connect your camera to the telescope focuser. Once you start introducing more optics such as the Barlow lens into your optical train, you dramatically reduce the amount of light reaching your camera sensor. As mentioned above, with a 2X Barlow for example, you will change the focal ratio on a f5 Newtonian, to f 10. Depending on the quality of the Barlow and guiding, you may also dramatically reduce image sharpness due to lens aberrations and increased magnification.
With the Barlow connected, you will have to place it at the right distance from the camera sensor to achieve focus. By moving it forward or closer to the camera in the T Adapter, you will have to find the right position, trial and error, as they say! If you want to zoom in, either crop your image in post processing or buy a telescope with a longer focal length.