Ahhh yes... the old dust bunnie problem. If you have a ccd camera then you better start getting used to them. Better quickly start to learn how to do flat fields. You use flat field for correcting the image for defects in the optical train like vignetting and dust bunnies.
btw, be careful blowing dust off your chip. use a camera puffer if you have one. Or a big syringe, but keep it well away from the chip surface. The second last thing you want on your chip is a spit bubble. Nasty when they dry

The last thing you want on your chip surface is a nice big scratch from the tip of the syringe


Goes without saying both syringe and blower need to be very clean inside.