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My calibration routine is
50 darks per exposure time I plan to use to generate master flats (Time consuming but I only do it once or twice a year)
100 bias as they don't take long to shoot and I re use the resulting master bias for months.
25 flats per filter per session. I shoot dawn sky flats and it happens so it is no bother to me. But with dawn flats you really need a good number so that dithering between shots allows for any visible stars in each flat to be rejected.
The flats I calibrate with the master bias to generate a master flat per filter per session that I can store with the appropriate lights.
The lights I calibrate with a bad pixel map, the master flats and either the master bias or the correct length dark. With the ASI294 bias frames were no good and it has a predictable glow so darks were essential but the ASI2600 (Both MC and MM) have essentially zero glow and good bias frames so I am using the master bias more and more now and darks less and less.
I shoot lots of bias and dark frames as it is a relatively small time investment in smooth masters that I can re use for months and months.
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