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Lack of dithering in the last shots I did with a 350D (The first ones where I could run to more than 30 seconds, so a toasty warm sensor) left measles in the resulting shots where darks subtracted out the hot pixels. They were hot as in max values so after dark subtraction there was no data left. COmbine that with guiding so the target was more or less stationary in frame and it really looked spotty.
I noticed less issues with the ASI294 but there was still an improvement by dithering, it was though a lot more subtle.
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