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I typically just shoot everything I can get as soon as a target is at least 30 degrees in elevation. Usually I am gathering data over several nights and discarding all bar my arbitrary number of best subs on the basis of any with distorted stars through wind gusts or other problems get the flick first, then the ones with the highest HFR get the flick, and if I still have enough, the ones with the brightest background go.
I have some subs shot recently over a night where it was 6-6-6 subs cycling through R-G-B through the night, when I have a chance I should plot the average HFR of each sub of each colour and see if there is a clear trend as the target rose higher.
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