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It would not be uncommon for any particular sensor to have hundreds of hot pixels (Remembering that this does not mean they will be at maximum value, just that they may end up brighter than the average pixel for the same exposure time) so 50 odd lines from hot pixels would not be out of the question.
They will not always show up in the same place on each image after stacking but if it is hot pixels they should be in the same places on the individual subs. The difference really is that from sub to sub the stars will wander around through tracking errors or dithering, but in the stack, the stars end up in the fixed locations and any hot pixels not rejected by the stacking software will wander around in the final image and make streaks, or dotted lines etc.
30 second exposures, are you guiding or imaging unguided? And do the stars move around on the individual subs in about the same way as these streaks do in the stacked image?
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