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I do 100 flats and 100 dark flats, the ASI294 is not the easierst camera in the word on calibration so I aim for the flats to be as high quality as I can, 100 averages the read noise very well.
I couldn't do it without a flat panel, I do my flats at ten seconds so sky flats would be a problem. The benefit of that is that I can reuse a master dark flat for a fairly long time as I know what my flat exposure time will be. I don't use bias frames, the ASI294 does not perform well with them due to peculiarities in it's setup.
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