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With the 533 you want the gain at 100 (which is also unity gain for the camera) - that's the point you get the biggest drop in read noise (from almost 3.5e to 1.5e), dynamic range restored to ~13.5 stops & maintain a good full well capacity. Higher gain will give minimal improvement to read noise at a huge cost to both dynamic range & full well capacity. And as Martin says, dither often.
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