I take flats every single time I image. I take them right after my imaging session with an EL sheet. At the same temperature. I also take 'dark flats' that I subtract from the 'light flats' to scale them. I do the dark flats at the same temperature and same duration as the light flats. For my flats regardless of the camera or filter I aim for an ADU between 9000 and 10000. I do about 80 lights and 100 darks.
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