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Stars look a little bloated to me. As you have identified it could be guiding, could be focus - but if it was me I'd look into that. Perhaps get a bahtinov mask if you don't have one.
Also don't know why you're using the in-camera noise reduction (which if I'm not mistaken basically takes an in-camera dark and subtracts it from the previous light frame). That's what dark subtraction in DSS (or whatever software you're using) is for. Not to mention that taking in camera darks after every light frame effectively halves your available imaging time. For me I'd much rather gather twice as many light frames
Why not just build up a 'library' of dark frames on an evening when you're not doing any imaging, note the ambient temps, and then use the appropriate dark frames or dark masters when it comes to processing? If the reason is hot pixels left after dark subtraction in DSS, you can get rid of those easily with the cosmetic correction feature.
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