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I may have been lucky, I had no hot pixels, and do most of my work at 1:1 and just didn't get an issue. I don't see any amp glow either, I make sure the amp is switched off in the software and that seems to do the job.
With the hyperstar I'm no longer doing really long exposures anymore either.
If darks are required then making a library of dark frames would be enough in most cases if required to get rid of read noise or other noise if you experience any on particular targets.
Of course flats and bias frames are another story.
The Quantix 6303 though is another beast altogether my god that thing is useless without a set of darks.
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