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The vertical grid spacing on the graph is 1 pixel on your guide camera. You need to work out the ASP for both the guide and imaging cameras.
For example I might get 0.79 ASP on the image camera at f/10 and 3.81 ASP on the guide camera. So I need to keep within 0.25 of the centre line to stay within 1 pixel on the image camera.
If I change imaging scopes I get 1.69 ASP at f/7.5, so then I only need to stay within 0.5 of the centre line.
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