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Thanks Clayton, yes the under exposure occurred when I was capturing the AVI file. I use IC Capture 2.1. The AVI file consisted of 3417 total frames and 1201 stacked frames with a low quality setting of 85% using Gradient2. The exposure time was 1/15 sec and the frame rate was 15fps. I forgot to change the "Hue" setting so I believe it was very low. Also I didn't know how to choose the proper gain setting to compensate for the reduction in light on the CCD sensor caused by inserting the 3X barlow. Which codec do you use? I was using RGB24 with the YUY2 color format.
Peter
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