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Nice Mark. Its a beautiful object and great rendition.
I don't think having your gain on zero would have hurt. Others may correct, but I believe the gain setting just "spreads out" the spectrum of values your CCD can record between 0 and full well depth. For example, if your sensor "fills up" at a 16bit value of 37,000, then by increasing the gain you get might spread the values out a bit further to say between 0 and 50,000. As long as you don't push the upper limit past 65,535 you don't lose anything and you spread the data over the available 16bit bandwidth a bit more.
On the other hand, "spreading" the data across the useable spectrum of values is what you're effectively doing in PS when you stretch.
Some people actually vow and declare gain of 0 is best to keep noise down. I normally run on 10/110 with my QHY9 for what its worth.
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