you can but the result is not as good as an averaged master dark. In built camera noise reduction will subtract the noise it sees in that particular exposure. If you get a cosmic ray strike during that partcular dark you end up with a messed up exposure.Taking several and avergaing gives a better and msoother view of the cameras dark noise.
Using in built darks subtraction also mean you waist valuable imaging time for each exposure while camera does noise reduction.
Regards
Chris
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