Hi Adam,
I use a Canon 450D, usually at ISO800 with subs of 3 to 5 minutes (depending on target brightness and focal length). To help manage thermal noise, I typically use 1min spacing between subs to allow the DSLR sensor to cool down a bit. If using short subs (eg. 2 to 3min) on a coolish night (below about 15C), I usually reduce the gap to about 30 seconds. Seems to suit the 450D.
Cheers,
Jeff
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