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Hi Peter, Keep your ISO. and every thing identical to capture except you can vary the exposure time. You shouldn't shift focus or camera or adapters etc as you are imaging the full imaging train and nothing else in this process. You may also find you get better results by also taking Bias frames and using them in the calibration process. Bias are easy. Just take as if you are taking darks at as close to the same temprature but at the fastest shutter speed available. This will reduce the read electronic noise from both your calibration files and your light files.
All pretty easy when you get it wored out and the improvement to your images will be imeadiately noticable.
Good luck with it all.
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