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Yes, as Alex points out, every time you move the camera (or anything else in the image train) new flats are required, and dust builds up over time, so the longer the time between sessions, the more likely you need new flats. If the rig is mobile, you need new flats after every session.
Photometrists on GRAS like to see new flats every week, even though the rigs are permanent.
I renew flats on my permanent home rig about every 5th session (the cap is on between times)
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