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Hi Chris,
Very nice image, love the colour and the detail in it would suggest that there is very little wrong with your collimation. Collimation is obviously critical at very high mags and any problem with it will smear the fine detail in images such as this.
I would suggest the temperature of your primary mirror and the quality of the seeing will be the most important thing that will contribute to any improvement on this image.
Very well done.
Regards
Trevor
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