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hi
i use the QHY5 on a WO80mm for guiding
i tend to use the dark frames as it usually improves contrast, you need to redo it everytime you fiddle with exposute time.
I think that in the "brain" settings i push gain to 100% not the sliding bar at the bottom of the main screen
As was mentionned earlier, focus is critical, if its out of focus a bit its ok but past a poit you really wont see the stars.
unlike others i use 0.2 sec exposures and i always manage to find a guide star, sometimes requiring me to re-aim the guide star.
I'd start off by lining it up on a real big bright star you just cant miss and using a Bahtinov or Hartmann mask ensure the focus is spot on.
cheers
frank
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