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Generally "subs" is referring to a collection of images taken to combine together to produce a single image either through adding, median combine, averaging or similar. Binning refers to on chip combining of pixels. Instead of using single pixels to catch the light they are "joined" together to make a super pixel. 2x2 binning combines 4 pixels into one. This give the advantage of increased sensitivity and greater well depth. The down side is it reduces your image size by 1/4.
From memory in CCDSoft a sub frame is a section of a frame being taken. It is normally smaller than the original and is used to get a faster download for focusing, or as you have done, for selecting only one area to be imaged (ROI region of interest). Strange though that it would give a larger image view. You don't have some form of auto-fit to window ticked somewhere do you?
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