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Leon re darks and temp.
For every 6 deg C increase in temp there is roughly a doubling of the temperature dependant dark noise, for every 6 dec C drop in temp the noise reduction is halved. So if you take a light at 30 deg and then a dark at 27 deg you could expect roughly (and this is roughly) 3/4 the amount of noise temperature dependent noise. This will show up in you subtractions.
There are other "dark" factors as well, but temperature is probably the most variable and has the greatest impact.
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