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I forgot to mention that darks were used. I made master darks for that evening at ISO 400, 800, 1000 and 3200, and also shot many subs at those sensitivities as well. As I have not got around to setting up the wedge, I'm getting about a 50% yield at 30 secs. Autoguiding is on the 'to do' list.
Normally I wouldn't even get the scope out under a bright moon and usually curse the moon as a thundering nuisance, however since getting the 40D I want to use it every clear night!
Here's the Tarantula later that evening at ISO 3200, 10 x 10 sec subs plus darks. More of a test to compare to lower ISO's. I hope to get around to processing the ISO 800 subs tomorrow.
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