To show the incredible difference cooling makes. Here is a version that was just aligned and stacked with no dark subtraction at all! Colour balance is a bit off, but where are the hot pixels? very few exist. I evened out the field with Gradient xterminator, that removed most of the amp glow too, but it wouldnot remove hot pixels. When I examined the 15 minute dark I also took, I could only see the odd "deviant pixels" those that are always hot regardless of temperature, the background of scattered coloured pixels are totally gone, its astounding. I reckon a 24 hour single image would have less noise than a 1 hour one at room temperature.
Scott
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