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Can you test it through the options dialogue successfully?
The times I've had problems are usually where my camera's battery is low, or DSLR Focus has been running for quite a while and other app's opened/closed etc, I think something sometimes gets confused with respect to the camera's connection. Oh, and Mirror lock-up has sometimes done odd things for me, so I don't usually use that.
I use a serial connection, sometimes converted through a Serial -> USB converter.
Steps I follow are pretty straight forward:
1) connect the camera and turn it on (manual mode, no mirror lock-up, manual focus).
2) load DSLR Focus
3) Connect to the camera
4) Focus
5) go to the long exposure tab
6) back on the main options window change the ISO.
7) set the long exposure details
8) start.
That usual works unless something wacky has messed it up (as mentioned above) in which case on those occasions I just reboot the PC if closing & re-opening DSLR Focus doesn't fix it.
Roger.
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