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I generally shoot sky flats, but with my cameras which are both ASI2600's, I configure the software for anything between 0.1 to 30 seconds at low gain (I actually use gain -20 with my pair of cams for flats and broadband subs) A master bias at the same gain and temp calibrates over that exposure range nicely.
I would not get too hung up on aiming for 1/3 on the histogram, my approach is to aim for around the 50% mark (so ADU of about 32K) but so long as the flat does not have pixels at zero or saturated after calibration then it goes in the pot to create the master flat.
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