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Nicely done, Carl. It's quite a step up from DSLR to astro-cam, and the learning curve does take some effort to get over.
It's my understanding that you need matching flat frames for each set of light frames. So for your L-Enhance light frames, you need a set of flat frames, and a further set of flat frames for the 'no filter' set of lights.
You can get away with one set of dark frames and one set of dark flats, as you're capturing any in-camera noise such as amp glow and thermal noise.
With the ASI294 (and the 183) you can't get reliable images under 3 seconds of exposure time. There's something up with these cameras that give you bad images. So capturing bias frames is not going to happen. Stick with ~5 second flats / dark-flats and you'll be fine.
Cheers,
V
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