Yep looks like Flexure to me.
Something that a lot people dont take into account...I was getting a fair bit of flexure and have improved the system via some tube rings on my OTA but I still have a little.
The flex can be coming from anywhere....Focuser, mount plates, guide rings, camera connection.I wouldn't be too concerned for 16 images thats not that bad.
Regarding exposure time 5minutes is ample..I would take more exposures and lower the ISO to reduce noise.
yes your dark frames should have reduced the amp glow more then it did.
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