Eric, the best way to remove dust is as Gary said, use the Sensor clean function which only raises the mirror. Then use a blower (No Brush) to gently puff the dust off the sensor without touching it. Failing that h0ughy has used some brushes specially designed for the task, hopefully he will post to this thread as well. There's nothing wrong with using the in camera long exposure noise reduction function. The 350D will then automatically take a dark of the same exposure time and subtract for you.
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