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Yes and no... longer subs will eventually warm the sensor as high as its going to get under the conditions. Which means the first few subs average temperature may be less than subsequent frames.
There are two ways of looking at the problem of getting reasonable average temperature consistency over subframes. The difference is that shorter cool down time increases the average temp over the subs compared with a longer cooling time - assuming that subs and delay are consistent and ambient air temp doesn't vary too much.
Some people advocate no delay whatsoever to keep the sensor at roughly the same temp throughout.
Take darks in the same way as the lights and if there are clouds, fit them in between holes to save time.
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