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As far as I know the longer the shutter is open the more light comes in, light hits the sensor at different times as well. I've seen it in the subs I have done when I got through and look for star tails. I can see certain pixels have lit up when in a previous sub it hadn't.
If my current set up could do 600 sec subs I'd be doing that. Alas, it's 60 sec subs for me.
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