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I've done focus stacking with my microscope, its great. Microscopes have such a tiny depth of focus its a great tool. I don't think its much usein astro though or using astro software as the stacking is different for different reasons. Focus stacking is about keeping the sharpest pixels from the stack and throwing the rest away. But Integration (astro stacking) keeps ALL the pixels to give you more depth/broader histogram in order to pull out faint signalswithout being swamped by noise. By averaging in integration you gain confidence for what each pixel should be which results in a visually sharper image than a single frame yields. But you throw away little of the data at all so to use it for focus stacking you retain all the good plus all the bad and the results are more an ortone or soft focus effect. Maybe artsy but not practical for the purpose, just because your cancer cells look nice does it serve any practical purpose?
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