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Yeah definitely clean. Flats remove dust spots but that's a huge one. Sometimes they won't if they are not just right.
Its far better to image with a clean camera and clean filters than rely on flats to remove dust spots in my experience.
So get yourself a rocket blower and some Bintel cleaning fluid and some Q tips and microfibre lens cleaning cloth with a bright torch (you need to shine the torch on the glass surfaces to reveal the dust quite often as it can be somewhat invisible otherwise).
That's my policy, cleaniness is senior to post processing flats etc.
Greg
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