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Acquiring the planet at high magnification on the tiny chip of a planetary camera is a common problem. It's probably not in the small field of view or your ROI, or if it is, it's way out of focus to the point of being barely visible. Focus with the cam is different from visual focus, and if you add the barlow it's different again.
Centre Jupiter in the finderscope. Put a low power eyepiece in the main scope and centre visually. Then a high power eyepiece (plus barlow if you're using one) and centre visually again. Then put in the ZWO and open Sharpcap. Most important is to start with maximum field of view setting (not a ROI) and the exposure and gain settings both racked up to near maximum.When you've acquired it, centre it again and then adjust gain, exposure and focus. If you then insert the barlow, you'll need to adjust focus again by quite a bit, and ramp up the exposure. Once you've got it centred and focussed with the barlow, then adjust the field of view setting down to a smaller ROI and your frame rate will increase significantly. Hope this helps.
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