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In your example, you need to consider the effect of sky glow (sky limited). As Shiraz has shown, paying attention to background ADU is important. Ray has also produced a set of exposure charts for the ASI1600 cameras, based on the sky quality in your location. Basically, the more sky glow the shorter the exposures, you simply shoot more, alot more, short subs to build the Signal. Of course as Rick has pointed out, the read noise of your camera is important in being able pull off this strategy. Thankfully the latest ultra low read noise CMOS cameras can work in this way.
As far as "pixels filling" is concerned, there are usually optimal well-depth settings for CMOS cameras. Pixels do not bleed.
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