Matt,
Backlash is movement in drive gears or worms. If a gear or a wormdrive is not a good fit it will have some backlash, which means it can move (although tiny, it is huge at the EP).
Focusers get backlash as well.
Picture this: You have just made a slight movement on your RA, Dec, or focuser and when you stop movement it jumps back a fraction, making centreing hard. That's backlash.
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Originally Posted by Matt
Any tips on what I should be aiming for when making these adjustments?
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Just make sure all gears are meshed properly.