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G'day Graham. I used in on a 40D and my current 5DII. Works fine on either.
That being said, this lens is specialised for macro only. It does not focus beyond say 100mm from front of lens. If you haven't done macro before, probably not the best to start with this one. And as Chris mentions, you usually need a flash to get enough light. Doesn't need to be twin light, but will need flash of some kind.
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