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Mick, the moons do not appear to be focused in the combined shot. There is no reason you can't use layering, this is common on very bright targets like M42 Orion nebula, where the core blows out when you expose for cloud detail. However, it is possible to get both the moons and Jupiter in one shot without layering, by using video frame stacking and exposing to at least pickup the moons. You do not mention how the image was acquired.
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