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The ideal length of subs and iso speed combination can vary between equipment and locations so you need to experiment a bit. In light polluted areas sub length will be much shorter before the background will be over exposed. Faster f ratio scopes will also work better with either shorter exposures or lower iso's . Temp wise sensor temps in the low 30's is not too bad for summer, mine has been running high 30's here in the tropics.
I check individual subs for focus (live view 10x zoom, then take a pic and zoom in on the image), background exposure and to see if I am getting some data of the subject before I start a sequence of subs. I check the focus every half hour or so because that can and does shift as the equipment cools.
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