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Having a similar number of darks is a good idea and yes, should be take under the same conditions. The good thing about them is that they can be used later on as well, for other shots.
Bias is very easy, just keep the same ISO and go with the quickest shutter speed, 1/8000 for my Nikon D700.
Flats are whatever exposure gives about a 50% histogram, I find that putting it on Aperture Priority makes this easy, just set my aperture as the same as lights (if using lens) or just on Programable (P)(Automatic) for a telescope.
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