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Hi Graham,
Really nice detail in your image mate, and Loving the colours. If you have PI there is another process called dynamic background extraction which is quite good for removing gradients etc. I copied your image and ran DBE on it a few times and then adjusted the histogram, and had to resize again after that coz it went over the 200k limit for the site. I have attached it below. There is a heap of tutorials on DBE around and when you do it properly it can take a while and a bit of mucking around to make sure samples are all in the right spots, but for this exercise I just let it choose it's own sample positions and ran it a few times. Hope that helps mate. PI has so many processes it's really hard to get your head around all of them.
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