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From tests I conducted a couple of years ago there was no difference when guiding 1x1 versus 2x2 in terms of accuracy providing SNR was ample. I wish I had kept the results but in summary how efficiently the software could calculate the star centroid was based on the SNR. 2x2 will often get you a good SNR using short exposures.
The calculation of multiple star centroids on a guide chip (multi-star guiding) will provide even greater guiding accuracy.
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