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Brent et al,
The ISO settings never impact on the number of photons received by the chip.
At ISO 100 and ISO 32000 the chip receives the same number of photons.
All the ISO does (in most cases) is the act as a multiplier to give a brighter image based on the photons received.
I find in spectroscopy, that ISO 200 to 400 is all you need,
Additional "gain" can be achieved with the imaging software.
Just my 2c
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