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Different ISOs won't be rejected, but you really need to stack them using the HDR method. Better to do all the same ISO and stack different ISOs in final post-processing (if you are seeking to capture a high dynamic range).
If they are slightly different durations, then right-click on the duration and change each of them all to the same value, e.g. 50 sec (DSS won't stack different exposure lengths, but you can trick it).
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