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I'm getting 90 secs or better from a 450D at 1600 ISO but ambient at night is about 12*-16*. A cooler box should get me down another 10* or more. Currently the sensor tends to stay about 24-25* after a few frames have been taken. 10* lower is another 75% noise reduction according to most information I've read.
35-45* must be cooking the sensor just about, noise will be a big issue. I'd seriously look at either a Peltier system or even a water\ice\glycol circulating system to get it down.
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